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Figure 5. Tooth phenotypes in laf/alk8 and snh/bmp7 zebrafish mutants. Wild-type and mutant embryos at 5 and 8 days of age were stained with Quercetin (100 µg/mL, Sigma) for 3 hrs so that developing pharyngeal teeth could be visualized. At 5 days post-fertilization (dpf), age-matched wild-type siblings exhibit 3 teeth on each cb5 cartilage (panel A), while snh/bmp7 mutants exhibit a single tooth (panel B). At 8dpf, wild-type siblings display 3 well-formed teeth on each of the bilateral cb5 cartilages (panel C),while zygotic laf/alk8 mutants exhibit one tooth that has attached to the cb5 cartilage, and another tooth appears arrested in development (panel D).
