Figure 2. Effect of antibody specific for the 1,25-(OH)₂D₃ membrane receptor (Ab99) on protein kinase C (PKC) activity of growth zone chondrocytes treated with 1,25-(OH)₂D₃ or analogue 3a. Confluent, fourth-passage growth zone chondrocytes were treated for 9 min with control media, 10^-8 M 1,25-(OH)₂D₃ (left panel) or analogue 3a (right panel) in the absence or presence of Ab99 or rabbit immunoglobulin G1. At harvest, PKC specific activity in the cell layer was determined. Data represent the mean ± SEM of 6 cultures from one of two experiments yielding comparable results. *P < 0.05, treatment vs. control; •p < 0.05, Ab99 vs. no Ab99.

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