Critical Reviews in Oral Biology & Medicine, Vol 4, 531-535, Copyright © 1993 by International & American Associations for Dental Research
Characteristics of protooncogene expression in A5 cells
E. Kousvelari and C. K. Yeh
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Stimulation of beta-adrenergic receptors by isoproterenol or addition of
8-BrcAMP rapidly and transiently induces the expression of the
protooncogenes, c-fos, and jun B, but not that of c-jun in A5 cells. These
results indicate that different intracellular pathways may operate within
the same cell for the induction of this group of early response genes. The
inducibility of c-fos and jun B genes by either isoproterenol of 8-BrcAMP
is transcriptionally regulated and accompanied by increases in their
respective products. Furthermore, both c-fos and jun B mRNA levels are
elevated at G0/G1 phase of the A5 cell cycle and are inducible by
isoproterenol or 8-BrcAMP at the different phases of the cell cycle. These
data further suggest a possible role of c-fos and jun B in A5 cell cycle.
