Critical Reviews in Oral Biology & Medicine, Vol 4, 415-419, Copyright © 1993 by International & American Associations for Dental Research

ARTICLES

Localization and characterization of a parotid Ca(2+)-dependent ecto-ATPase

F. J. Dowd, L. S. Li, J. E. Campbell and P. H. Cheung
Department of Pharmacology, School of Medicine, Creighton University, Omaha, NE 68178.

Parotid acini were isolated and tested to further establish the presence of ecto-ATPase in the intact cells. Inhibitors were used to determine if the inhibitor profile of the ATPase was similar to that of a Ca(2+)-ATPase from parotid membranes identified previously as an ecto-ATPase. The Ca(2+)-ATPase of intact cells was insensitive to oligomycin (10 micrograms/ml), N-ethylmaleimide (NEM) (0.1 mM), ruthenium red (0.1 mM), sodium azide (1 mM), and was inhibited approximately 22% by sodium orthovanadate (Na3VO4) (1 mM). This profile was similar to the Ca(2+)-ATPase of intact cells. Trifluoperazine (TFP) (0.1 mM) inhibited the enzyme in intact cells by approximately 32%. The nucleotide substrate specificity of the enzyme also reflected very closely the pattern seen in isolated membranes.

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