Figure 4. Immunoreactivity of human fibronectin with antibodies directed against bacterial GroEL and human HSP60. (Lane 1) A. actinomycetemcomitans GroEL (0.02 µg). (Lane 2) Human plasma fibronectin (1.67 µg). (Lane 3) Fibroblast fibronectin (0.02 µg). (Lane 4) E. coli GroEL (0.01 µg). (Lane 5) Human plasma fibronectin (1.67 µg). (Lane 6) Human HSP60 (0.01 µg). (Lane 7) Human plasma fibronectin (1.67 µg). Lanes 1–3 were probed with pAb-AaGroEL. Lanes 4 and 5 were probed with pAb-EcGroEL. Lanes 6 and 7 were probed with mAb-HuHSP60.