Figure 8. (a) Dot blot showing interaction between iodoplatinate (IP) and malachite green-aldehyde (MGA) and major phospholipids (PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine; SM, sphyngomyelin) (lanes 1 and 5). Lane 2 demonstrates that IP staining is not affected by pre-treatment with acetone (AC) but disappeared (lane 3) after pre-treatment with chloroform-methanol (Ch-m). Four amino-acid-bearing functions that may interfere with IP staining were unstained (lane 4). Lane 5 shows the staining obtained with MGA: PS and SM are stained, whereas only a thin rim is observed for PC, and PE is unstained. Dentin phosphoprotein (PP) is unstained. (b) The distribution of MGA-stained matrix components in rat incisor predentin (pd). Aggregates appear as granules and filaments in intercollagen spaces. X140,000. (c) IP-stained matrix components in rat incisor predentin. They are also located in intercollagen spaces. Collagen (Co) appears as electron-lucent areas. X140,000.